Real-time PCR (qPCR) is a useful and robust method of quantifying gene expression, provided that suitable reference genes are used to normalize the data.To date, suitable reference genes have not been validated for tomato gene expression changes in response to Tomato curly stunt virus (ToCSV).RT-qPCR was conducted on resistent (R) and susceptible (
In recent years, there has been a decline in the efficiency of coal mining by traditional methods.On the one hand, the volume of coal outside the contour of the cut, which is economically Duct Cover Kit impractical for open-pit mining, is increasing.On the other hand, when using underground geotechnology, the cost of production exceeds the cost of
Abstract Background The last malaria cases in near-elimination settings are often found in international border Chews regions due to the presence of hard-to-reach populations, conflict, uneven intervention coverage, and human migration.Test-and-treat border posts are an under-researched form of active case detection used to interrupt transmission c